Effect of maize root exudates on indole-3-acetic acid production
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چکیده
It is assumed that plant growth regulators produced by beneficial Background bacterial species could also influence plant growth. IAA is a major plant growth regulator responsible for stimulation of plant growth. There are several microorganisms which are naturally responsible for Ltryptophan metabolism. : In total, 56 indigenous morphologically distinct isolates from rice Methods roots were selected and subsequently characterized with biochemical tests, 16S rRNA sequencing and plant growth promoting activities. Pseudomonas RE1 (GenBank: MF102882.1) and RE17 (GenBank: MF103672.1) fluorescens endophytes resulted in better PGP activity against the other 54 isolates. Both endophytes were tested to screen indole-3-acetic acid production ability in pure culture conditions with L-tryptophan at 0, 50, 100, 200 and 500μg/ml concentrations. : RE1 was recorded efficient for indole production in Results P. fluorescens comparison to RE17 at various L-tryptophan concentrations. P. fluorescens P. RE1 was shown to produce between 0.8 μg/ml and 11.5μg/ml of fluorescens indole at various tryptophan concentrations, while RE17 produced between 1.2μg/ml and 10.2μg/ml. At 200 and 500μg/ml tryptophan concentration, P. RE17 produced 7.4pmol/ml and 9.3pmol/ml IAA, respectively. fluorescens : Inoculation of maize seed with RE1 and RE17 Conclusions P. fluorescens showed a significantly higher level of IAA production in comparison to non-inoculated seeds. Current study outcomes proved that plant growth regulators produced by Pseudomonas species could also play a critical role in plant growth promotion. Arun Karnwal ( ) Corresponding author: [email protected] : Conceptualization, Data Curation, Formal Analysis, Investigation, Methodology, Supervision; : Resources Author roles: Karnwal A Dohroo A No competing interests were disclosed. Competing interests: Karnwal A and Dohroo A. How to cite this article: Effect of maize root exudates on indole-3-acetic acid production by rice endophytic 2018, :112 (doi: bacteria under influence of L-tryptophan [version 1; referees: awaiting peer review] F1000Research 7 ) 10.12688/f1000research.13644.1 © 2018 Karnwal A and Dohroo A. This is an open access article distributed under the terms of the Copyright: Creative Commons Attribution , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Licence The author(s) declared that no grants were involved in supporting this work. Grant information: 25 Jan 2018, :112 (doi: ) First published: 7 10.12688/f1000research.13644.1 1
منابع مشابه
Effect of maize root exudates on indole-3-acetic acid production
It is assumed that plant growth regulators produced by beneficial Background bacterial species could also influence plant growth. IAA is a major plant growth regulator responsible for stimulation of plant growth. There are several microorganisms which are naturally responsible for Ltryptophan metabolism. : In total, 56 indigenous morphologically distinct isolates from rice Methods roots were se...
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Background: It is assumed that plant growth regulators produced by beneficial bacterial species could also influence plant growth. IAA is a major plant growth regulator responsible for stimulation of plant growth. There are several microorganisms which are naturally responsible for L- tryptophan metabolism. Methods: In total, 56 indigenous morphologically distinct isolates from rice roots were ...
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